Aquaporin-4 (AQP4) (NMO-IgG) Antibody, CBA with Reflex to Titer

• Test Details
• Technical Information
• CPT Coding
• Specimen Requirements
• Shipping Considerations

Test Details

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Test code:	1282
Type of disorder:	Multiple Sclerosis
Disease(s) tested for:	Neuromyelitis Optica, Demyelinating Disease, Myelin Disorder
Profiles that contain this test:	NMO Spectrum Evaluation (AQP4, CBA reflex to MOG, CBA)
Special Notes:	If the AQP4 antibody, CBA is positive a semi-quantitative titer will be performed at an additional charge (CPT code 86052).

Technical Information

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Clinical Significance:	NMO (neuromyelitis optica, Devic disease) is an immune-mediated chronic inflammatory disease that predominantly affects the optic nerve and spinal cord and presents with optic neuritis (ON) and myelitis. Limited forms of this disease, such as isolated ON, brainstem encephalitis and longitudinal extensive transverses myelitis (LETM), have been referred to as NMO spectrum disorders (NMOSD). NMO was originally thought to be a variant of multiple sclerosis, but is now recognized as a distinct disease. The presence of serum antibodies against aquaporin-4 (AQP4) is associated with NMO and NMOSD.
Methodology:	Cell-based Immunofluorescence Assay
Reference Range:	See Laboratory Report

CPT Coding

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The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

CPT:

86052

Specimen Requirements

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Please label each specimen tube with two forms of patient identification. These forms of identification must also appear on the requisition form.

Specimen Type:	Serum
Specimen Stability:	Room temperature: 72 hours, Refrigerated: 30 days, Frozen: 30 days
Specimen Requirements:	2 mL (0.5 mL minimum) serum in a Red-top tube or Serum Separator Tube (SST)
Instructions:	Serum must be separated from whole blood within 48 hours of collection. Please label each specimen tube with two forms of patient identification. These forms of identification must also appear on the requisition form.
Alternate Specimen Type:	Cerebrospinal Fluid (CSF)
Alternate Specimen Stability:	Room temperature: 72 hours, Refrigerated: 30 days, Frozen: 30 days
Alternate Specimen Requirements:	2 mL (0.5 mL minimum) CSF collected in a plastic screw cap vial

Shipping Considerations

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Transport Temperature:	Refrigerated
Reject Criteria:	Turbid and/or any suggestion of bacterial contamination
Set-up/Analytic Time:	3-7 days